Proliferation of myogenic stem cells in human skeletal muscle in response to low-load resistance training with blood-flow restriction

摘要

Low-load resistance training with blood-flow restriction has been shown to elicit substantial increases in muscle mass and muscle strength; however the effect on myogenic stem cells (MSC) and myonuclei number remains unexplored. Ten male subjects (22.8±2.3 yrs) performed 4 sets of knee extensor exercise (20% 1RM) to concentric failure during blood-flow restriction (BFR) of the proximal thigh (100 mmHg), while eight work-matched controls (21.9±3.0 yrs) trained without BFR (CON). 23 training sessions were performed within 19 days. Maximal isometric knee extensor strength (MVC) was examined pre and post training, while muscle biopsies were obtained at baseline (Pre), after 8 days intervention (Mid8) and 3 (Post3) and 10 days (Post10) post training to examine changes in myofibre area (MFA), MSC and myonuclei number. MVC increased by 7.1% (Post5) and 10.6% (Post12) (P